Pedosphere 18(6): 731--740, 2008
ISSN 1002-0160/CN 32-1315/P
©2008 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Changes of bacterial community structure in copper mine tailings after colonization of reed (Phragmites communis) |
CHEN Yu-Qing 1, REN Guan-Ju2, AN Shu-Qing2, SUN Qing-Ye3, LIU Chang-Hong2 and SHUANG Jing-Lei2 |
1 College of Life Science, Nanjing Normal University, Nanjing 210046 (China). E-mail: chenyuqing@njnu.edu.cn;
2 The State Key Laboratory of Pollution Control and Resource Reuses, School of Life Science, Nanjing University, Nanjing 210093 (China);
3 School of Life Science, Anhui University, Hefei 230039 (China) |
| ABSTRACT |
| Soil samples were collected from both bare and vegetated mine tailings to study the changes in bacterial communities and soil chemical properties of copper mine tailings due to reed (Phragmites communis) colonization. The structures of bacterial communities were investigated using culture-independent 16S rRNA gene sequencing method. The bacterial diversity in the bare mine tailing was lower than that of the vegetated mine tailing. The former was dominated by sulfur metabolizing bacteria, whereas the latter was by nitrogen fixing bacteria. The bare mine tailing was acidic (pH = 3.78), whereas the vegetated mine tailing was near neutral (pH = 7.28). The contents of organic matter, total nitrogen, and ammonium acetate-extractable potassium in vegetated mine tailings were significantly higher than those in the bare mine tailings (P < 0.01), whereas available phosphorus and electrical conductivity were significantly lower than those in the bare mine tailings (P < 0.01). The results demonstrated that 16S rRNA gene sequencing could be successfully used to study the bacterial diversity in mine tailings. The colonization of the mine tailings by reed significantly changed the bacterial community and the chemical properties of tailings. The complex interactions between bacteria and plants deserve further investigation. |
| Key Words: 16S rRNA gene, bacterial diversity, bacterial community, functional group, mine tailings |
| Citation: Chen, Y. Q., Ren, G. J., An, S. Q., Sun, Q. Y., Liu, C. H. and Shuang, J. L. 2008. Changes of bacterial community structure in copper mine tailings after colonization of reed (Phragmites communis). Pedosphere. 18(6): 731-740. |
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