Pedosphere 18(1): 86--91, 2008
ISSN 1002-0160/CN 32-1315/P
©2008 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Enhancement of NH4+ uptake by NO3- in relation to expression of nitrate-induced genes in rice (Oryza sativa) roots |
ZHAO Xue-Qiang 1,2, ZHAO Shou-Ping1,2 and SHI Wei-Ming1 |
1 State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008 (China). E-mail: xqzhao@issas.ac.cn;
2 Graduate School of the Chinese Academy of Sciences, Beijing 100049 (China) |
| ABSTRACT |
| This study aimed to survey the expression of genes involved in rice N uptake and aasimilatory network and to understand the potential molecular mechanisms responsible for the NO3-enhanced NH+ uptake. By using quantitative real-time polymerase chain reaction (PCR), the genes related to N nutrition, including ammonium transporters (AMTs) and ammonium assimilatory enzymes (GS and GOGAT), were transcriptionally analyzed in rice plants grown in the absence and presence of NO3- in the NH4+-containing medium. The results showed that NH4+ uptake by rice was enhanced by the NO<sup>3 supply to the medium. At the same time and in parallel, the amount of transcripts of seven genes (OsAMT1;1, OsAMT1;2, OsAMT4;1, OsGLN2, OsGLU1, OsGLT1, and OsGLT2) was increased in rice roots, but the expression of two genes (OsGLN1;1 and OsGLN1;2) was decreased and that of OsAMT1;3 remained without change. Upor down-regulation of these genes involved in NH<sup>+ uptake and assimilation correlated with the increase in NH4+ uptake in the presence of NO3- in rice roots. |
| Key Words: ammonium, gene expression, nitrate, quantitative real-time PCR, rice roots |
| Citation: Zhao, X. Q., Zhao, S. P. and Shi, W. M. 2008. Enhancement of NH4+ uptake by NO3- in relation to expression of nitrate-induced genes in rice (Oryza sativa) roots. Pedosphere. 18(1): 86-91. |
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