Pedosphere 17(1): 36--43, 2007
ISSN 1002-0160/CN 32-1315/P
©2007 Soil Science Society of China
Published by Elsevier B.V. and Science Press
| Nodule formation and development in soybeans (Glycine max L.) in response to phosphorus supply in solution culture |
MIAO Shu-Jie1,2,3 , QIAO Yun-Fa1, HAN Xiao-Zeng1 and M. AN4 |
1 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081 (China). E-mail: miaoshujie@126.com;
2 Key Laboratory of Soybean Biology of Ministry of Education, Harbin 150030 (China);
3 Graduate School of the Chinese Academy of Sciences, Beijing 100049 (China);
4 E. H. Graham Centre for Agricultural Innovation, NSW Department of Primary Industries&Charles Sturt University, Wagga Wagga, NSW2650 (Australia) |
| ABSTRACT |
| Phosphorus (P) is necessary for growth and nitrogen fixation, and thus its deficiency is a major factor limiting legume production in most agricultural soils. The effect of phosphorus supply on nodule development and its role in soybeans (Glycine max L.) was studied in a nutrient solution. Plants were inoculated with Bradyrhizobium japonicum and grown for 35 days in a glasshouse at a day and night temperature of 25 ℃ and 15 ℃, respectively. Although increasing P supply increased the concentrations of P and N in the shoots and roots, the external P supply did not significantly affect the P concentration in the nodules, and the N fixed per unit nodule biomass decreased with increasing P supply. The nitrogen content in the shoots correlated well with the P content (r = 0.92**). At an inoculation level of 102 cells mL-1, the P supply did not affect the number of nodules; however, at inoculation levels of 103.5 and 105 cells mL-1, increasing P supply increased both the number and size of nodules. Irrespective of the inoculation level, increasing P supply increased the nodule biomass relative to the biomass of the host plant. It is suggested that the P deficiency specifically inhibited the nodule development and thereby the total N2 fixation. |
| Key Words: inoculation level, nitrogen fixation, nodulation, phosphorus, soybeans |
| Citation: Miao, S. J., Qiao, Y. F., Han, X. Z. and An, M. 2007. Nodule formation and development in soybeans (Glycine max L.) in response to phosphorus supply in solution culture. Pedosphere. 17(1): 36-43. |
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