Elsevier

Pedosphere

Volume 30, Issue 4, August 2020, Pages 563-569
Pedosphere

Microbial remediation of a pentachloronitrobenzene-contaminated soil under Panax notoginseng: A field experiment

https://doi.org/10.1016/S1002-0160(17)60476-4Get rights and content

Abstract

Pentachloronitrobenzene (PCNB) is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop seedlings. Microbial removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues. A short-term field experiment (1 year) was conducted to explore the potential role of a PCNB-degrading bacterial isolate, Cupriavidus sp. YNS-85, in the remediation of a PCNB-contaminated soil on which Panax notoginseng was grown. The following three treatments were used: i) control soil amended with wheat bran but without YNS-85, ii) soil with 0.15 kg m-2 of solid bacterial inoculum (A), and iii) soil with 0.30 kg m-2 of solid bacterial inoculum (B). The removal of soil PCNB during the microbial remediation was monitored using gas chromatography. Soil catalase and fluorescein diacetate (FDA) esterase activities were determined using spectrophotometry. In addition, cultivable bacteria, fungi, and actinomycetes were counted by plating serial dilutions, and the microbial biodiversity of the soil was analyzed using BIOLOG. After 1 year of in-situ remediation, the soil PCNB concentrations decreased significantly by 50.3% and 74.2% in treatments A and B, respectively, when compared with the uninoculated control. The soil catalase activity decreased in the presence of the bacterial isolate, the FDA esterase activity decreased in treatment A, but increased in treatment B. No significant changes in plant biomass, diversity of the soil microbial community, or physicochemical properties of the soil were observed between the control and inoculated groups (P < 0.05). The results indicate that Cupriavidus sp. YNS-85 is a potential candidate for the remediation of PCNB-contaminated soils under P. notoginseng.

REFERENCES (40)

  • D Lièvremont et al.

    Removal of PCNB from aqueous solution by a fungal adsorption process

    Water Res

    (1998)
  • C S Liu et al.

    Oxidative degradation of propachlor by ferrous and copper ion activated persulfate

    Sci Total Environ

    (2012)
  • Y Liu et al.

    Effects of amorphous-zinc-silicate-catalyzed ozonation on the degradation of p-chloronitrobenzene in drinking water

    Appl Catal A Gen

    (2011)
  • F Sannino et al.

    Pesticide influence on soil enzymatic activities

    Chemosphere

    (2001)
  • H W Wen et al.

    Application of gamma irradiation in ginseng for both photodegradation of pesticide pentachloronitrobenzene and microbial decontamination

    J Hazard Mater

    (2010)
  • A Abubakar et al.

    Tributyltin (TBT) tolerance of indigenous and non-indigenous bacterial species

    Water Air Soil Pollut

    (2016)
  • P K Arora et al.

    Degradation of chlorinated nitroaromatic compounds

    Appl Microbiol Biotechnol

    (2012)
  • S Fontaine et al.

    Size and functional diversity of microbe populations control plant persistence and long-term soil carbon accumulation

    Ecol Lett

    (2005)
  • J L Garland et al.

    Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization

    Appl Environ Microbiol

    (1991)
  • T Gorontzy et al.

    Microbial degradation of explosives and related compounds

    Crit Rev Microbiol

    (1994)
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