Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China

doi:10.1016/S1002-0160(19)60840-4

Pedosphere

Volume 30, Issue 1, February 2020, Pages 73-86

https://doi.org/10.1016/S1002-0160(19)60840-4 Get rights and content

Abstract

Denitrification is one of the major processes causing nitrogen loss from arable soils. This study aimed to investigate the responses of nirS-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast China. Soil samples were collected from sites located in the north (NB), middle (MB), and south (SB) of the black soil region of Northeast China, each with four chronic fertilization regimes: no fertilizer (NoF), chemical fertilizer (CF), manure (M), and chemical fertilizer plus manure (CFM). Methods of quantitative polymerase chain reaction (qPCR) and Illumina MiSeq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nirS gene. The results showed that the M and CFM regimes significantly increased the abundances of nirS-type denitrifiers compared with NoF at the three locations. The majority of nirS sequences were grouped as unclassified denitrifiers, and the different fertilizers induced little variation in the relative abundance of known nirS-type denitrifier taxa. Over 90% of the sequences were shared among the four fertilization regimes at each location, but none of the abundant operational taxonomic units (OTUs) were shared among the three locations. Principal coordinate analysis (PCoA) revealed that the communities of nirS-type denitrifier were separated into three groups that corresponded with their locations. Although similar fertilization regimes did not induce consistent changes in the nirS-type denitrifier communities, soil pH and NO⁻₃-N content simultaneously and significantly influenced the structure of nirS-type denitrifier communities at the three locations. Our results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nirS-type denitrifier community structures, and similar chronic fertilization regimes did not induce consistent shifts of nirS-type denitrifier communities in the black soils.

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Excreta addition reduced the Shannon index of nirS and nirK, as shown in Table 4. Excreta serves as a source of N, and the addition of N had a negative effect on the Shannon index (Wang et al., 2018; Hu et al., 2020) possibly because N addition reduced the abundance of rare species, resulting in decreases in microbial richness. However, some research found that organic materials increased the diversity of nirS (Tao et al., 2018; Huang et al., 2020).
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Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China

Abstract

Eur J Soil Biol

Soil Biol Biochem

Soil Biol Biochem

Trends Microbiol

Soil Biol Biochem

Agric Ecosyst Environ

Pedosphere

J Biol Chem

Soil Biol Biochem

Appl Soil Ecol

Appl Soil Ecol

Soil Biol Biochem

Appl Soil Ecol

Soil Biol Biochem

Sci Total Environ

FEMS Microbiol Ecol

FEMS Microbiol Ecol

Eur J Soil Biol

Bioresour Technol

Soil Biol Biochem

Global change, nitrification, and denitrification: A review

Glob Biogeochem Cycles

Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil

Biogeochemistry

Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N2O reduction, isolated from soda lakes

Microbiology

Impact of land use management and soil properties on denitrifier communities of Namibian savannas

Microb Ecol

QIIME allows analysis of high-throughput community sequencing data

Nat Methods

Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil

Microb Ecol

Immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria

Appl Environ Microbiol

Insights into the effect of soil pH on N2O and N2 emissions and denitrifier community size and activity

Appl Environ Microbiol

Diversity and distribution of sediment nirS-encoding bacterial assemblages in response to environmental gradients in the eutrophied Jiaozhou Bay, China

Microb Ecol

Halomonas mongoliensis sp. nov. and Halomonas kenyensis sp. nov., new haloalkaliphilic denitrifiers capable of N₂O reduction, isolated from soda lakes

Immunological identification and distribution of dissimilatory heme cd₁ and nonheme copper nitrite reductases in denitrifying bacteria

Insights into the effect of soil pH on N₂O and N₂ emissions and denitrifier community size and activity