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15N NMR Spectroscopic Study on Nitrogen Forms in Humic Substances of SoilsEnglish Full Text

WEN QIXIAO, ZHUO SUNENG and CHENG LILI (Institute of Soil Science, the Chinese Academy of Sciences, P.O. Box 821, Nanjing 210008 (China))

Abstract: Nitrogen forms of humic substances from a subalpine meadow soil, a latentic red soil and a weathered coal and the effect of acid hydrolysis on N structures of soil humic substances were studied by using 15N cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS NMR) spectroscopy. Of the detectable 15N-signal intensity in the spectra of soil humic substances 71%-79% may be attributed to amide groups, 10%-18% to aromatic/aliphatic amines and 6%~11% to indole- and pyrrole-like N. Whereas in the spectrum of the fulvic acid from weathered coal 46%, at least, of the total 15N-signal intensity might be assigned to pyrrole-like N, 14% to aromatic/aliphatic ammes, and the remaining intensities could not be assigned with certainty. Data on nonhydrolyzable residue of protein-sugar mixture and a 15N-labelled soil fulvic acid confirm the formation of nonhydrolyzable heterocyclic N during acid hydrolysis. Project (No. 39790100) supported by the National Natural Science Foundation of China.
  • Series:

    (D) Agriculture

  • Subject:

    Fundamental Science of Agriculture; Agronomy

  • Classification Code:

    S153.6

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